Reduced rhizosphere colonization ability ofAgrobacterium tumefaciens chromosomal virulence (chv) mutants |
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Authors: | Martha C. Hawes Steven G. Pueppke |
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Affiliation: | (1) Department of Plant Pathology and Molecular and Cellular Biology, University of Arizona, 85721 Tucson, AZ, USA;(2) Department of Plant Pathology, University of Missouri, 65211 Columbia, MO, USA |
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Abstract: | Mutations in the chromosomal virulence (chv) region ofA. tumefaciens strain A723 reduce virulence, motility, and ability of the bacteria to bind to plant cells. We conducted experiments to assess the ability ofchv mutants to colonize the rhizosphere ofPisum sativum. The mutation had no effect on ability of bacteria to grow with a defined number of root cap cells as the sole carbon and nitrogen source. Ten days after inoculation, there were up to 103-fold more wild type thanchv mutant bacteria present in the rhizosphere of inoculated plants. |
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Keywords: | Agrobacterium tumefaciens binding Pisum sativum rhizosphere root cap cells |
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