Purification and some properties of proliferation suppressing factor from human lung adeno carcinoma PC-8 |
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| Authors: | Hiroshi Shinmoto Shun'ichi Dosako Koji Yamada Sanetaka Shirahata Hiroki Murakami |
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| Affiliation: | (1) Technical Research Institute, Snow Brand Milk Products Co. Ltd., 1-1-2 Minamidai, 350 Kawagoe, Japan;(2) Institute of Food Science and Technology Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, 812 Fukuoka, Japan;(3) Graduate School of Gene Resources Technology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, 812 Fukuoka, Japan |
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| Abstract: | Extracts by 3 M KCl from a human lung adenocarcinoma cell line, PC-8, suppressed the proliferation of phytohemagglutinin activated lymphocytes. The proliferation suppressing facfor (PSF) was purified from the extracts by ammonium sulfate precipitation, gel filtration and ion exchange chromatography. The purified PSF had a molecular weight of 50 kDa by SDS-PAGE and was acid-and heat-labile. Since the PSF also suppressed the proliferation of three established human cell lines, it is unlikely that the PSF hinders the interaction between lymphocytes and mitogens. |
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| Keywords: | PC-8 PHA activated lymphocytes proliferation suppression |
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