Isotope Discrimination by Ribulose 1,5-Diphosphate Carboxylase: No Effect of Temperature or HCO(3) Concentration |
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Authors: | Christeller J T Laing W A |
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Institution: | Plant Physiology Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand. |
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Abstract: | Carbon 13 isotope discrimination by ribulose 1,5-diphosphate carboxylase from soybean (Glycine max Merr.] cv. Amsoy) was studied as a function of temperature, bicarbonate concentration, and pH. None of these factors affected the degree of discrimination against 13C. The average δ13C was −28.3%, a value close to that found for whole C3 plants. The zero temperature response observed here with ribulose 1,5-diphosphate carboxylase corroborates data from whole plants. The lack of effect of bicarbonate concentration on discrimination is consistent with both current theories of alternate forms of carboxylase. |
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