|
|||||
|
|
A reliable method for the use of oligonucleotides as probes in blot-hybridization experiments |
|
| Authors: | Jean Paquette Carmen Sapienza |
| Institution: | (1) Ludwig Institute for Cancer Research, 687 Pine Avenue West, H3A 1A1 Montreal, Quebec, Canada;(2) Present address: Ludwig Institute for Cancer Research, Department of Medicine, University of California, San Diego, 9500 Gilman Drive, 92093-0660 La Jolla, California, USA |
| Abstract: | We have developed a ligation and specificprimer radiolabeling method that allows the use of oligonucleotides as probes in blot-hybridization experiments. The major advantage of the protocol is that standard hybridization and washing conditions may be used and yield high signals and low background. The observed increase in the stability and intensity of the hybridization signals appears to result from both increased length and specific radioactivity of the hybridization probe. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |