杨梅苷脂质体的制备研究

目的：制备杨梅苷脂质体。方法：采用逆相蒸发法制备杨梅苷脂质体。用冷冻离心法分离脂质体和游离药物,用高效液相色谱法测定药物含量并计算包封率。采用激光粒度仪测定平均粒径。结果：杨梅苷脂质体制备的最佳处方和工艺为：卵磷脂：杨梅6：1,卵磷脂：胆固醇2：1,有机相：水相4：1,磷酸盐缓冲溶液的pH值为6.86,浓度为0.005 mol.L-1;超声时间为5分钟。结论：最佳条件下制备的杨梅苷脂质体包封率较高,粒径分布好,质量稳定。

Study on the Preparation of the Myricitrin Liposome

Objective： To prepare the myricitrin liposome. Methods： Myricitfin liposome was prepared by reverse-phase evaporaton technique; The liposomesand free drug was separated by refrigerated centrifugation, then the concentration of myricitrin was determined by HPLC and the liposome entrapment efficiency was calculated. The particle size was determined by particle size analyzer. Results： The optimum formulations and the preparation technology were phospholipids： myricitrin =6：1; phospholipids： cholesterin=4：1; oil-phase： water phase=4：1 （volume ratio）; the buffer pH value was 6.86 and the buffer concentration was 0.005 mol·L^-1; the ultrasound time was 5 minutes. Conclusions： The myricitrin liposome with the optimal formulation and preparation had a high entrapment efficiency, good particle size distribution and stable quality.