| 蛋白转导域透膜作用的研究进展 |
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| 引用本文： | 汤莹,史道华.蛋白转导域透膜作用的研究进展[J].生物磁学,2009(14):2756-2758,2762. |
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| 作者姓名： | 汤莹 史道华 |
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| 作者单位： | [1]福建医科大学福总医学院药学科,福建福州350108 [2]南京军区福州总医院药学科,福建福州350025 |
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| 基金项目： | 福建省自然科学基金项目（No.2008J0103）;南京军区医学科学技术研究“十一五”计划课题（06MA154） |
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| 摘 要： | HIV-TAT蛋白转导域（Protein transduction domain，，PTD）是新近发现的一种在蛋白转导过程中能高效穿过生物膜的结构域，源自人类免疫缺陷病毒Tat蛋白的一段碱性氨基酸多肽，能与多肽、蛋白质及DNA等分子连接并跨膜导入绝大部分的组织细胞或透过血脑屏障，转导效率高且对细胞无损伤。TAT-PTD与细胞膜之间的电荷作用，吸附于膜表面，依赖脂筏介导的巨胞饮作用进入细胞。TAT融合蛋白系统是一种极有价值的运载工具，在基础医学研究和临床治疗方面有着广泛的应用前景。
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| 关 键 词： | 蛋白转导域 人免疫缺陷病毒反式激活蛋白 融合蛋白传递 透膜作用 |
Progress in Inchylosis of Protein Transduction Domain |
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| TANG Ying,SHI Dao-hua.Progress in Inchylosis of Protein Transduction Domain[J].Biomagnetism,2009(14):2756-2758,2762. |
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| Authors: | TANG Ying SHI Dao-hua |
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| Institution: | 1 Department of Pharmacy, Fuzong Medical school of Fujian Medical University, Fuzhou 350025, China ;2 Department ofPharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China) |
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| Abstract: | HIV-TAT protein transduction domain （PTD）, a base peptide of tat protein discovered recently from human immunodeficiency virus type I （HIV-1）, can transfer protein across cell membrane in most tissues or across blood-brain barrier by binding with peptides, protein or DNA with high efficiency and no cytotoxicity. Protein transmembrane by TAT-PTD is associated with lipid-raft-mediated macropinocytosis by initial-ionic interaction. TAT fusion protein system is a very valuable delivery tool and widely used for the medical research and clinical application in diseases treatment. |
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| Keywords: | Protein transduction domain Human immunodeficiency virus trans-activator protein Fusion protein transfer Transmembrane |
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