华蟾素对子宫内膜癌HEC-1-B细胞株凋亡和增殖的影响

目的：探讨华蟾素对体外培养子宫内膜癌HEC-1-B细胞凋亡、增殖以及侵袭能力的影响。方法：体外培养HEC-1-B细胞，经不同浓度华蟾素（0．2mg／ml、2mg／ml和20mg／m1）干预24h后，采用MTT法观察细胞生长情况，流式细胞术分析细胞凋亡，Transwell小室检测细胞体外侵袭能力。结果：华蟾素能有效抑制HEC．1．B细胞生长，诱导细胞凋亡。未经华蟾素处理的细胞凋亡率仅为2．5％，经0．2mg／ml、2mg／ml、20mg／ml的华蟾素作用24h后，细胞凋亡率分别为7．4％、44．3％和78．5％。趋势卡方检验x^2=165．4983，P〈0．0001。HEC-1-B细胞经华蟾素作用后，细胞侵袭能力降低。在高浓度时，华蟾素有可能存在细胞毒作用。结论：华蟾素能通过诱导HEC-1-B细胞凋亡，从而抑制HEC-1-B细胞生长，并且降低细胞的侵袭能力。

The Effect of Cinobufacini on Apoptosis and Growth of Endometrial Cancer HEC-1-B Cell Line

Objective： To investigate the effect of cinobufacini on the apoptosis, growth, and invasion capacity of the endometrial cancer HEC-1-B cells cultured in vitro. Methods： Endometrial cancer HEC-1-B cells were cultured in vitro. After the action of different concentrations （0.2 mg/ml, 2 mg/ml, and 20 mg/ml） of cinobufacini, the growth of HEC-1-B cells was measured by MTT colorimetry. Apoptosis was tested by flow cytometry. The invasion capacity of HEC-1-B cells treated with cinobufacini was detected with transwell chamber model. Results： The cinobufacini effectively inhibited the growth and invasion of HEC-1-B cells, and induced cell apoptosis. The apoptotic rate of HEC-1-B cells not treated with cinobufacini was only 2.5 %, while the apoptotic rate increased to 7.4 %, 44.3 %, and 78.5 %, respectively, after the HEC-1-B cells were exposed to cinobufacini for 24 hours in different concentrations af 0.2 mg/ml, 2 mg/ml, and 20 mg/ml. Statistic analysis showed that the increment was significant （x^2=165.4983, P 〈0.0001）. Cinobufacini showed to be cytotoxic at the high concentration. Conclusion： Cinobufacini can inhibit the growth and invasion capacity of HEC-1-B cells by means of inducing apoptosis of the cells.