TNFa-308基因启动子多态性与新疆维吾尔族人乙型肝炎易感性的相关性研究

目的：研究TNF-α基因单核苷酸多态性（SNP）308G—A位点与新疆地区维吾尔族人乙型肝炎之间的关系。方法：以聚合酶链式反应－限制性内切酶长度多态性（PCR-RFLP）技术，对120例乙肝患者和120例正常对照者TNF—α基因SNP308多态性位点进行基因分型。结果：SNP308多态性位点G／G基因型和G／A基因型频率在病例组为77％和23％，正常对照组为88％和12％，2组基因型和等位基因频率分布差异有显著性（p〈0．05）。结论：TNF—α基因启动子308多态性位点与新疆维吾尔族人乙肝有明显相关性。

Study on the relationship between the genetic polymorphisms of TNFa Promoter and susceptibility of viral hepatitis B in XinJiang Uigur Population

Objective： To investigate the correlation between single nucleotide polymorphisms （SNP308） of TNFa gene and viral hepatitis B in XinJiang Uigur Population. Method： A 1：1 Matched Case-control Study was conducted with 120 cases of viral hepatitis B and 120 population-based controls. TNFa gene polymorphisms genotypes were detected by PCR-RFLP. Result： The distribution of G/G and G/A genotype of the the TNFa at position -308 were 88%and 12% in hepatitis B patients,whereas 77% and 23% in the controls. The frequencies of alleles and genotypes of TNFa at position-308 in hepatitis B patients were significantly different from those of the controls （p〈0.05）. Conclusion： The results suggest that the TNFa308 gene were associated with viral hepatitis B in XinJiang Uigur Population.