应用基因芯片筛选鼻咽癌信号转导相关基因

目的：建立具有组织特异性的鼻咽癌基因表达谱，筛选鼻咽癌中信号转导相关基因。方法：采用深圳微芯公司基于玻片的包含8046个人类基因的基因芯片，检测7例鼻咽癌组织及1例鼻咽炎组织，初步获得鼻咽癌异常表达基因；结合GO分类从异常表达的基因中筛选信号转导相关基因，以Biocarta信号通路数据库查询筛选基因相关转导信号通路信息。结果：在鼻咽癌组织独得1241个异常用表达基因，其中高表达基因871个，低表达基因343个。发现28个差异表达基因与细胞的信号转导相关，其中表达上调的21个，表达下调的7个。结论：成功建立了具有组织特异性的鼻咽癌基因表达谱，初步获得了鼻咽癌信号转导相关基因。

Screening Signal Transduction -Regulated Genes for Nasopharyngeal Carcinoma Using Gene Chips

Objective： To screen signal transduction -regulated genes in nasopharyngeal carcinoma using gene chips. Methods： A preliminary selection of abnormal expression genes from the gene chips of the Shenzhen Weixin company which detected the seven nasopharyngeal carcinoma and one rhinopharyngitis. And then to integrate the data of signal transduction -regulated by Gene Ontology, screen signal transduction -regulated genes from gene chips in NPC from BioCarta. Result： 1.In the basic of gene chips, there are 871 high expression genes and 343 low expression genes in the nasopharyngeal carcinoma tissue. 2.There are 27 differential expression relate gene of signal conducting. 21 of them are high expressing and 7 are low express. 3.We accepted 7 signal transduction -regulated genes from BioCarta which could play important role in the development of NPC. And we got several signal pathways. Conclusion： We found a new way how to screen signal transduction-regulated genes from gene chips in NPC and then make a preliminary identification of the Candidate Gene.