Immune cytolysis of human malignant melanoma by antibody to oncofetal antigen I (OFA-I) |
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| Authors: | N Sidell R F Irie D L Morton |
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| Institution: | (1) Division of Oncology, Department of Surgery, 54-140 CHS UCLA School of Medicine, University of California, 90024 Los Angeles, California, USA;(2) Surgical Service, Sepulveda Veterans Administration Hospital, 91343 Sepulveda, CA, USA |
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| Abstract: | Summary Oncofetal antigen I (OFA-I) has been identified by immunofluorescence and immune adherence (IA) as a membrane antigen on human tumor cells, which cross-reacts with fetal brain tissue. This antigen induces humoral antibody in patients with cancer. The present work was designed to evaluate the complement-dependent cytotoxic potential of anti-OFA-I antibody produced in melanoma patients against an OFA-I-positive melanoma cell line, UCLA SO M14 (M14). Patients' sera were chosen on the basis of anti-OFA-I activity by IA. Alloantibodies to M14 were removed by absorption of the sera with lymphoblastoid cells autologous to M14. Fourteen sera were tested, and all demonstrated cytotoxic activity in the presence of rabbit complement. Human complement was also shown to mediate cytotoxicity, although less effectively than rabbit complement. The specificity of the reaction was confirmed when the cytotoxic capability could be absorbed by fetal brain, but not by autologous fetal liver tissues. These results indicate that a patient's serum antibody to OFA-I can lyse tumor cells expressing this antigen. |
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