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| 鸡下丘脑cDNA文库的构建及部分克隆ESTs序列初步分析 | |
| 引用本文： | 赵志辉,李宁,刘兆良,张玉静,吴常信.鸡下丘脑cDNA文库的构建及部分克隆ESTs序列初步分析[J].遗传学报,2001,28(4):301-305. |
| 作者姓名： | 赵志辉 李宁 刘兆良 张玉静 吴常信 |
| 作者单位： | 1. 中国农业大学;中国人民解放军军需大学生物化学教研室 2. 中国农业大学 3. 中国人民解放军军需大学生物化学教研室, 4. 中国农业大学动物科技学院, |
| 基金项目： | 国家杰出青年科学基金资助(批准号：39725022)；国家重大基础研究发展规划项目(批准号：G20000161) |
| 摘 要： | 以鸡下丘脑为实验材料，以λgt10为载体，构建了鸡下丘脑cDNA文库。结果表明，文库的滴度为3.8×10 |
| 关 键 词： | 鸡 下丘脑 cDNA文库 表达序列标签 功能基因 |
| 文章编号： | 0379-4172(2001)04-0301-05 |
| 修稿时间： | 2000年7月17日 |
Construction of Chicken Hypothalamus cDNA Library and Primary Analysis of ESTs Sequences from Partial Clones |
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| ZHAO Zhi-Hui,LI Ning,LIU Zhao-Liang,ZHANG Yu-Jing,WU Chang-Xin.Construction of Chicken Hypothalamus cDNA Library and Primary Analysis of ESTs Sequences from Partial Clones[J].Journal of Genetics and Genomics,2001,28(4):301-305. | |
| Authors: | ZHAO Zhi-Hui LI Ning LIU Zhao-Liang ZHANG Yu-Jing WU Chang-Xin |
| Institution: | ZHAO Zhi-Hui,LI Ning,LIU Zhao-Liang1,ZHANG Yu-Jing,WU Chang-Xin |
| Abstract: | Using hypothalamus as material and λgt10 as vector, chicken hypothalamus cDNA library has been constructed. The results showed that the titration of the cDNA library we constructed was 3.8×106, and the fragment length of inserted DNA ranged mainly from 1.0～3.0kb. The ESTs was sequenced and primarily analyzed for ten clones randomly selected from above library. After analysis with BLASTn and FASTA3.1, it was found that four ESTs developed matched homologous sequence in chicken, four also had similar sequences in human and other species, have other two sequences were function-unknown gene. The sequences of ten ESTs we studied have been loaded into GenBank |
| Keywords: | chicken hypothalamus cDNA library expressed sequence tags (ESTs) |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |