Cloning and characterization of the Chlamydomonas moewusii mitochondrial genome. |
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Authors: | Robert W. Lee Carole Dumas Claude Lemieux Monique Turmel |
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Affiliation: | (1) Department of Biology, Dalhousie University, B3H 4J1 Halifax, Nova Scotia, Canada;(2) Déparement de biochimie, Faculté des sciences et de génie, Université Laval, GIK 7P4 Québec, Québec, Canada |
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Abstract: | Summary We report that the mitochondrial genome of Chlamydomonas moewusii has a 22 kb circular map and thus contrasts with the mitochondrial genome of Chlamydomonas reinhardtii, which is linear and about 6 kb shorter. Overlapping restriction fragments spanning over 90% of the C. moewusii mitochondrial DNA (mtDNA) were identified in a clone bank constructed using a Sau3AI partial digest of a C. moewusii DNA fraction enriched for mtDNA by preparative CsCI density gradient centrifugation. Overlapping Sau3AI clones were identified by a chromosome walk initiated with a clone of C. moewusii mtDNA. The mtDNA map was completed by Southern blot analysis of the C. moewusii mtDNA fraction using isolated mtDNA clones. Regions that hybridized to C. reinhardtii or wheat mitochondrial gene probes for subunit I of cytochrome oxidase (cox1), apocytochrome b (cob), three subunits of NADH dehydrogenase (nadl, nad2 and nad5) and the small and the large ribosomal RNAs (rrnS and rrnL, respectively) were localized on the C. moewusii mtDNA map by Southern blot analysis. The results show that the order of genes in the mitochondrial genome of C. moewusii is completely rearranged relative to that of C. reinhardtii. |
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Keywords: | Chlamydomonas moewusii Mitochondrial DNA Map Gene localization Evolution |
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