| Abstract: | Protein A molecular thin film was fabricated as a platform of antibody-based biosensor. For the immobilization of the protein
A thin film, a viologen multilayer was built up using the Langmuir-Blodgett (LB) technique, and then, protein A was adsorbed
on the viologen LB film by an electrostatic interaction force, which was formed as a hetero-film structure. For the deposition
of viologen, surface pressure area (π-A) isotherm was investigated. The fabricated protein A-viologen hetero LB film was investigated
using atomic force microscopy (AFM). Using the developed molecular film, antibody immobilization and fluorescence measurement
was carried out. |
