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Babesia bovis: Genome size, number of chromosomes and telomeric probe hybridisation |
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| Authors: | Shirley H Jones Ala E Lew Wayne K Jorgensen Stephen C Barker |
| Institution: | a Department of Parasitology, University of Queensland, Brisbane, Queensland 4072, Australia b Animal Research Institute, Yerongpilly, Queensland 4105, Australia c Tick Fever Research Centre, Wacol, Queensland 4076, Australia d Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Queensland 4072, Australia |
| Abstract: | Pulsed field gel electrophoresis of intact chromosomes of Babesia bovis revealed four chromosomes in the haploid genome. A telomere probe, derived from Plasmodium berghei, hybridised to eight SfiI restriction fragments of genomic B. bovis DNA digests indicating the presence of four chromosomes. A small subunit (18S) ribosomal RNA gene probe hybridised to the third chromsome only. The genome size of B. bovis is estimated to be 9.4 million base pairs. The sizes of chromosomes 1, 2, 3 and 4 are estimated to be 1.4, 2.0, 2.8 and 3.2 million base pairs, respectively. |
| Keywords: | Author Keywords: Babesia bovis genome chromosome telomere ribosomal |
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