The plant cell inhibitor KRP6 is involved in multinucleation and cytokinesis disruption in giant-feeding cells induced by root-knot nematodes |
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Authors: | Paulo Vieira Janice de Almeida Engler |
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Institution: | 1Lab. Nematologia/ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas; Universidade de Évora; Núcleo da Mitra; Évora, Portugal;2Institut National de la Recherche Agronomique; UMR 1355 ISA/Center National de la Recherche Scientifique; UMR 7254 ISA/ Université de Nice-Sophia Antipolis; UMR ISA; Sophia-Antipolis, France |
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Abstract: | The plant cell cycle inhibitor gene KRP6 has been investigated in roots infected by plant-parasitic root-knot nematodes (Meloidogyne spp.). Unexpectedly, KRP6 overexpressing lines revealed a distinct role for this specific KRP as an activator of the mitotic cell cycle. This function was confirmed in Arabidopsis thaliana suspension cultures ectopically expressing KRP6. A blockage in the mitotic exit was observed in cell suspensions and in giant cells resulted in the appearance of multi-nucleated cells. KRP6 expression during nematode infection and the similarity in phenotypes among KRP6 overexpressing cell cultures and giant-cell morphology strongly suggest that KRP6 is involved in multinucleation and acytokinesis occurring in giant-cells. Once again nematodes have been shown to manipulate the plant cell cycle machinery in order to promote gall establishment. |
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Keywords: | arabidopsis thaliana cyclin dependent kinase giant cells kip-related proteins mitosis meloidogyne incognita |
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