Arabidopsis PME17 Activity can be Controlled by Pectin Methylesterase Inhibitor4 |
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| Authors: | Fabien Sénéchal Alain Mareck Paulo Marcelo Patrice Lerouge Jér?me Pelloux |
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| Affiliation: | 1EA3900-BIOPI Biologie des Plantes et Innovation, Université de Picardie Jules Verne; Amiens, France;2EA4358-GlycoMEV Glycobiologie et Matrice Extracellulaire Végétale; IFRMP 23; UFR des Sciences et Techniques; Université de Rouen; Mont-Saint-Aignan, France;3ICAP Plateforme d’Ingénierie Cellulaire et Analyses des Protéines; Université de Picardie Jules Verne; Amiens, France |
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| Abstract: | The degree of methylesterification (DM) of homogalacturonans (HGs), the main constituent of pectins in Arabidopsis thaliana, can be modified by pectin methylesterases (PMEs). Regulation of PME activity occurs through interaction with PME inhibitors (PMEIs) and subtilases (SBTs). Considering the size of the gene families encoding PMEs, PMEIs and SBTs, it is highly likely that specific pairs mediate localized changes in pectin structure with consequences on cell wall rheology and plant development. We previously reported that PME17, a group 2 PME expressed in root, could be processed by SBT3.5, a co-expressed subtilisin-like serine protease, to mediate changes in pectin properties and root growth. Here, we further report that a PMEI, PMEI4, is co-expressed with PME17 and is likely to regulate its activity. This sheds new light on the possible interplay of specific PMEs, PMEIs and SBTs in the fine-tuning of pectin structure. |
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| Keywords: | Arabidopsis thaliana cell wall co-expression growth pectin pectin methylesterase pectin methylesterase inhibitor subtilase root |
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