Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients |
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| Authors: | Lourdes Fernández-Delgado Antonio Vega-Rioja Inmaculada Ventura Cristina Chamorro Rocío Aroca Manuel Prados Pedro Bobadilla David Rodríguez Ricardo Palacios Javier Monteseirín |
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| Institution: | 1 Unidad de Gestión Clínica de Alergia Intercentros, Hospital Universitario Virgen Macarena, Sevilla, Spain, ; 2 Departamento de Medicina. Facultad de Medicina. Universidad de Sevilla, Sevilla, Spain, ; 3 Servicio de Alergia, Hospital Infanta Elena, Badajoz, Spain, ; 4 Laboratorios Diater, Madrid, Spain, ; The Hospital for Sick Children and The University of Toronto, CANADA, |
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| Abstract: | BackgroundDespite the evidence that Lactoferrin (Lf) is involved in allergic asthma processes, it is unknown whether neutrophils can be one of the main cellular sources of this key inflammatory mediator directly in response of an IgE mediated stimulus. The present study was undertaken to analyze this question.MethodsNeutrophils from healthy subjects (n = 34) and neutrophils from allergic asthmatic patients (n = 102) were challenged in vitro with specific allergens to which the patients were sensitized, PAF, or agonist mAbs against IgE-receptors, and the levels of Lf were measured in the culture supernatant. The levels of serum IgE together with the severity of symptoms were also analyzed.ResultsLf was released into the culture supernatant of neutrophils from allergic asthmatic patients in response to allergens and PAF. This response was highly allergen-specific, and did not happen in neutrophils from healthy donors. Allergen effect was mimicked by Abs against FcεRI and galectin-3 but not by FcεRII. The levels of released Lf correlated well with the levels of serum specific IgE and severity of asthma symptoms. These observations represent a novel view of neutrophils as an important source of Lf in allergic asthma. Importantly, the levels of released Lf by neutrophils could therefore be used to evaluate disease severity in allergic asthmatic patients. |
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