Isolation of Rickettsia felis in the Mosquito Cell Line C6/36 |
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| Authors: | Maurício C Horta Marcelo B Labruna Edison L Durigon and Teresinha T S Schumaker |
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| Institution: | Maurício C. Horta, Marcelo B. Labruna, Edison L. Durigon, and Teresinha T. S. Schumaker |
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| Abstract: | We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene). |
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