Modification methylase M.Sau3239I from Streptomyces aureofaciens 3239 |
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| Authors: | E Zelinková M Paulícek J Zelinka |
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| Affiliation: | Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Czechoslovakia. |
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| Abstract: | By chromatography on phosphocellulose and Heparin-Sepharose the modification methylase M.Sau3239I was detected and partly purified from cells of Streptomyces aureofaciens 3239. Methylation by this enzyme protects DNA from cleavage by the restriction endonuclease R.Sau3239I. The enzyme catalyzes methylation of adenine to N-6-methyladenine in the 5'-CTCGmAG-3' recognition sequence. |
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