Combined frequency- and time-domain NMR spectroscopy. Application to fast protein resonance assignment |
| |
Authors: | Bernhard Brutscher |
| |
Affiliation: | Institut de Biologie Structurale - Jean-Pierre Ebel, UMR5075 CNRS-CEA-UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France. Bernhard.Brutscher@ibs.fr |
| |
Abstract: | A simple and general method is presented to simplify multi-dimensional NMR spectra of isotope-labeled bio-molecules. The approach is based on band-selective Hadamard-type frequency encoding, which disperses the correlation peaks into different sub-spectra. This makes it possible to apply low-dimensionality-based NMR techniques to larger molecular systems. Here we demonstrate the use of band-selective Hadamard frequency labeling for fast protein resonance assignment, based on our recently proposed suite of 2D experiments (Bersch et al., 2003). |
| |
Keywords: | GFT Hadamard spectroscopy protein resonance assignment reduced dimensionality |
本文献已被 PubMed SpringerLink 等数据库收录! |
|