肥大细胞释放生物活性介质促进TNCB致敏淋巴细胞分泌IL-17

氯化苦咪酸(TNCB)是诱导接触性超敏反应(CHS)实验模型的常用试剂,IL-17参与CHS的致病过程。利用TNCB致敏C57BL/6小鼠,4d后无菌分离淋巴结细胞。同时制备并体外活化同源小鼠成熟骨髓来源的肥大细胞(BMMC),成熟的BMMC具有肥大细胞特异性表型(FcεRI+/c-kit+),活化后可分泌TNF-α和IL-6等生物活性介质。在抗原提呈细胞存在下,活化的BMMC与淋巴结细胞体外共同培养72h,结果显示,与未致敏淋巴细胞共同培养组相比,BMMC与TNCB致敏淋巴细胞的共同培养上清中IL-17分泌水平显著增高(P0.01)。由此提示,活化的肥大细胞通过释放生物活性介质,促进TNCB致敏淋巴细胞IL-17的分泌。

Release of Bioactive Media by Mast Cell to Promote TNCB-Sensitized Lymphocytes to Excrete IL-17

Trinitrochlorobenzene （TNCB） is one of the common regents to induce contact hypersensitivity （CHS） experimantal model, and interleukin （IL）-17 participates the pathogenic process of CHS. C57BL/6 mice were sensitized with TNCB and the lymphocytes from the draining lymph nodes were separated sterilely 4 days after the sensitization. At the same time mature bone marrow-derived mast cells （BMMC） from homologous mice were prepared and activated in vitro. The mature BMMC possessed specific phenotypes （FcεRI＋/c-kit＋） of mast cells, and after the activation the BMMC excreted tumor necrosis factor （TNF）-α and IL-6 and other bioactive media. Moreover, under the existence of antigen presenting cells, the activated BMMC and lymphocytes from lymph nodes were cultured together in vitro for 72 hours, the results showed that as compared with the ones that not co-cultured with non-sensitized ones the level of IL-17 in the supernatant of co-cultural BMMC with TNCB-sensitized lymphocytes was increased significantly （P0.01）. Suggesting that the activated BMMC could promote TNCB-sensitized lymphocytes to induce the excretion of IL-17 through release of bioactive media.