Nucleotide sequence of the thioredoxin gene fromEscherichia coli |
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Authors: | Jan-Olov Höög Hedvig von Bahr-Lindström Staffan Josephson Betty J Wallace Sidney R Kushner Hans Jörnvall Arne Holmgren |
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Institution: | (1) Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden;(2) Department of Chemistry, KabiGen AB, S-112 87 Stockholm, Sweden;(3) Department of Molecular and Population Genetics, University of Georgia, 30602 Athens, GA, USA |
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Abstract: | The nucleotide sequence of the thioredoxin gene fromEscherichia coli was determined. The structural gene was identified on a cloned 3-kbPvuII Iragment by hybridization with a synthetic oligodeoxyribonucleotide corresponding to a part of the amino acid sequence of thioredoxin. Restriction-enzyme fragments were used as templates in the dideoxy sequence method, directly and after subcloning into M13mp8. A segment of 450 nucleotides was determined using both strands7 alternatively, without extensive overlaps. The sequence contains the thioredoxin coding region, a potential ribosome-binding site, and a putative promotor region. The predicted amino acid sequence differs by two inversions from the previously given thioredoxin sequence. The revised sequence is presented and the results further show that thioredoxins fromE. coli B and K12 are identical. |
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