High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy |
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Authors: | Verveer Peter J Swoger Jim Pampaloni Francesco Greger Klaus Marcello Marco Stelzer Ernst H K |
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Institution: | Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. verveer@mpi-dortmund.mpg.de |
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Abstract: | We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens. |
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