黑腹果蝇3号染色体裂翅平衡致死位点的发现及2号、3号染色体裂卷翅双平衡染色体的建立

果蝇的平衡染色体在遗传研究中被广泛应用.文章通过分析黑腹果蝇裂翅新突变体与野生型、982紫眼及黑檀体杂交后代裂翅性状情况,首次将裂翅基因定位于3号染色体上,并阐明了裂翅平衡致死、杂合子纯繁的遗传机制,获得了以裂翅为显性标记的3号平衡染色体品系.探索了双平衡染色体显性标记基因聚合的杂交模式,成功建立了以裂翅和卷翅为标记的2号、3号双平衡染色体.裂翅的发现为3号染色体平衡子提供了更加方便识另0的显性翅型标记,同时裂卷翅双平衡体的建立丰富了果蝇常用工具平衡子,可以广泛用于基因定位及突变筛选过程.

A new balancer of chapped wing on chromosome 3 and a gathering line with double balancers of chapped wing (L) and Cy in Drosophila melanogaster.

The balancers of Drosophila melanogaster are widely used in genetic research. By analyzing the phenotype of offspring from hybridization of chapped wing (L) mating with OR, 982p and e, respectively, we mapped the chapped wing mutation on chromosome 3 for the first time and demonstrated the chapped wing mutation as a new balancer of D. melanogaster with dominant wing nicking phenotype. Finally, we bred a novel gathering line with double balancers of L and Cy in D. melanogaster. The mutant L provided a legible dominant marker for the balancer of chromosome 3, and the cultivation of double balancers chapped-curly wing enriches the balancer stock, which is often used in mapping and screening.