Reduced rhodopsin phosphorylation during retinal dystrophy |
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| Authors: | D J Takemoto J Cunnick L J Takemoto |
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| Affiliation: | 1. Department of Radiology, NYU Langone Health, New York, New York;2. Department of Biomedical Engineering, University of California Davis, Davis, California;3. Department of Ophthalmology, NYU Langone Health, New York, New York;1. Department of Ophthalmology, Kobe City Eye Hospital, Kobe, Japan;2. Department of Ophthalmology, Toho University Graduate School of Medicine, Tokyo, Japan;3. Department of Ophthalmology, Kobe City Medical Center General Hospital, Kobe, Japan;4. Department of Surgery, Division of Ophthalmology, Kobe University Graduate School of Medicine, Kobe, Japan;5. Vision Care Inc., Kobe, Japan |
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| Abstract: | During inherited retinal dystrophy in Irish Setter dogs, decreased activity of cGMP phosphodiesterase (PDE) results in high cGMP levels and retinal degeneration (1-3). This defect could be in PDE itself, or in its interactions with other proteins of the rod outer segment. We report herein that when retinas from 8-week-old dogs were phosphorylated with gamma-32P-ATP, and separated on SDS-PAGE, phosphorylation of rd dog rhodopsin was reduced. When rd retinas were mixed with normal dog retinas, phosphorylation of the latter was inhibited. Since rd-mediated inhibition was prevented by 1 mM NaF, the results suggest that the cause of reduced rd phosphorylation is increased phosphatase activity. Together, these results demonstrate that decreased phosphorylation of rhodopsin due to increased phosphatase activity is a fundamental biochemical change which may partially account for the degenerative process and loss of visual acuity during inherited retinal dystrophy. |
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