Isolation and properties of reduced nicotinamide adenine dinucleotide phosphate-hepatoredoxin reductase of rabbit liver mitochondria.

An NADPH-hepatoredoxin reductase was purified from mitochondria of rabbit hepatocytes. The optical absorption spectrum showed a typical flavoprotein. The NADPH-hepatoredoxin reductase has an FAD as a coenzyme and the molecular weight of the NADPH-hepatoredoxin reductase was estimated to be 51000 by SDS-polyacrylamide gel electrophoresis. The NADPH-hepatoredoxin reductase was immunochemically similar to NADPH-adrenodoxin reductase of bovine and pig adrenocortical mitochondria, but not NADPH-cytochrome P-450 reductase of rabbit liver microsomes. The NADPH-cytochrome c reductase activity of the NADPH-hepatoredoxin reductase and hepatoredoxin complex, unlike NADPH-cytochrome P-450 reductase, was decreased by increasing ionic strength.