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Strand separation of DNA fragments and their isolation from nondenaturing polyacrylamide gels |
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| Authors: | Robert James Ralph A. Bradshaw |
| Affiliation: | Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110 USA |
| Abstract: | A technique for rapidly and quantitatively denaturing double-stranded DNA employing urea and moderate heat is described. The single DNA strands are resolved on high-percentage nondenaturing polyacrylamide gels from which they can be recovered for Maxam-Gilbert sequence analysis. |
| Keywords: | strand separation Maxam-Gilbert sequencing urea porcine relaxin cDNA gel electrophoresis nucleic acids |
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