Preprophase bands in cultured multinucleate soybean protoplasts |
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Authors: | H Wang A J Cutler L C Fowke |
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Institution: | (1) Department of Biology, University of Saskatchewan, Saskatoon;(2) Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan |
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Abstract: | Summary Multinucleate cells derived from soybean protoplasts were used to investigate the effect of increased nuclear number on the development and frequency of preprophase bands (PPBs) of microtubules (MTs). The results do not support the assumption that one nucleus establishes one PPB because the majority of multinucleate cells had only one large PPB. However, nuclear number or ploidy level has some influence on PPB development since double PPBs occurred more often in multinucleate than uninucleate cells. Double (divergent) PPBs were present at early and late stages of PPB development, suggesting that they are not a transient stage. PPBs in multinucleate cells developed in a similar fashion to those in uninucleate cells. In multinucleate cells, each dividing nucleus had its own spindle and phragmoplast. Subsequent phragmoplast development was frequently uncoupled from PPB distribution. Most multinucleates contained a single large PPB but at telophase, multiple phragmoplasts oriented in different planes.Abbreviations MT
microtubule
- MtSB
microtubule stabilizing buffer
- PBS
phosphate buffered saline
- PNF
perinuclear fluorescence
- PPB
preprophase band |
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Keywords: | Microtubule Nuclear number Preprophase band Soybean protoplast |
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