Two carbocyclic locked nucleic acid analogues give structural information about the role of hydration in A-type duplexes |
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Authors: | Albaek Nanna Petersen Michael Nielsen Poul |
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Affiliation: | Nucleic Acid Center, Department of Chemistry and Physics, University of Southern Denmark, DK-5230 Odense M, Denmark. |
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Abstract: | Two locked nucleic acid (LNA) analogues with three-carbon 2'-4' linkages, saturated or unsaturated, are synthesized using a ring-closing metathesis based strategy. Strongly stabilized duplexes with complementary RNA and slightly destabilized duplexes with complementary DNA are observed. CD-spectroscopy indicates a less pronounced shift toward A-type duplexes compared to LNA. These results combining a strong N-type conformation with the absence of a 2'-oxygen demonstrate a stronger importance of minor groove hydration in an intermediate duplex type than in an A-type duplex. |
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