Immuno-detection of aluminium and aluminium induced conformational changes in calmodulin - implications in Alzheimer's disease |
| |
Authors: | Levi Raphael Wolf Tamar Fleminger Gideon Solomon Beka |
| |
Institution: | (1) Ecotoxicology Division, Industrial Toxicology Research Centre, Post Box 80, M.G. Marg, Lucknow, 226 001, India |
| |
Abstract: | Studies were conducted to ascertain any involvement of free radical mediated prooxidative processes in different brain regions following diazopam administration. A significant decrease in TBA reactive substance formation was observed in cerebral cortex, cerebellum and brain stem regions after single doses of 1.5, 3 and 6 mg/kg b.wt. For further studies rats were given diazepam (i.p.) at 3 mg/kg body weight dose and sacrificed after 1 h to follow changes in the pro/antioxidant status. An enhancement in the TBARS formation was found in the mitochondrial fractions from cerebral cortex and brain stem. This effect was highest in brain stem being 107% as compared to controls. In the post mitochondrial fraction, cerebellum showed 49% enhancement whereas decreased formation of thiobarbituric acid reactive substances was observed in cerebral cortex and brain stem. Isozymes of superoxide dismutase showed a decrease in activity which was region dependent. Even though, total thiols were not significantly altered, free thiols showed depletion in cerebellum (39.8%) and brain stem (50%). Glutathione reductase activity was also decreased in cerebellum and brain stem. The results indicate that a single dose of diazepam causes free radical mediated changes and the modulatory response of antioxidant defences appears to be region specific. |
| |
Keywords: | diazepam free radicals antioxidants rat brain |
本文献已被 SpringerLink 等数据库收录! |
|