RNA polymerase subunit biosynthesis in Bacillus subtilis |
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Authors: | Randell T Libby and Lyle R Brown |
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Institution: | (1) Department of Microbiology, Oregon State University, 97330 Corvallis, Oregon, USA;(2) Present address: Department of Microbiology, The Ohio State University, 484 West 12th Avenue, 43210 Columbus, Ohio, USA |
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Abstract: | Summary The relative rates of RNA polymerase biosynthesis in Bacillus subtilis has been examined under steady-state growth conditions. The synthesis of RNA polymerase subunits (, , , ) has been followed by subunit fractionation of immunoprecipitated 3H]-labelled samples on SDS-polyacrylamide gels. The stoichiometries of ::: subunits have been determined from cultures pulse-labelled during steady-state growth. The results suggest that an unassembled pool of the -subunit exists from which the holoenzyme is formed.Upon shift-up from acetate to glycerol containing medium, a rapid rise in the differential rate of core enzyme synthesis was observed, while the rate of synthesis of the -subunit was not stimulated. During shift-down, a concomitant reduction in the rate of synthesis of all subunits occurred for the first 20 min after the shift; thereafter, a rate of synthesis characteristic of the new growth rate was established.As cultures enter sporulation, an immediate reduction in the rate of -subunit synthesis was demonstrated. |
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