The primary processes of photosystem II in purified guard-cell protoplasts and mesophyll-cell protoplasts from Commelina communis L. |
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Authors: | Michael F. Hipkins Peter J. Fitzsimons Jonathan D. B. Weyers |
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Affiliation: | 1. Biochemistry Section, National Vegetable Research Station, Wellesbourne, CV35 9EF, Warwick, UK
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Abstract: | The performances of four gas chromatographic detectors (nitrogen-phosphorus, flamephotometric, electron-capture and flame-ionisation) were compared for the analysis of permethylated cytokinins. The nitrogen-phosphorus and flame-photometric detectors (selective for nitrogen and sulphur-containing compounds respectively) gave greatly enhanced sensitivity and-or specificity compared with the flame-ionisation detector for a wide range of cytokinin types. The response of the electron-capture and flame-ionisation detectors was quantitatively similar. The improved performance of the nitrogen-phosphorus and flame-photometric detectors was used to develop rapid and simple purification techniques for the assay of zeatin and zeatin riboside in coconut milk; dihydrozeatin and dihydrozeatin riboside in bean leaves and cytokinin nucleosides in wheat-germ transfer RNA. |
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