Transcription profiling of fertilization and early seed development events in a solanaceous species using a 7.7 K cDNA microarray from Solanum chacoenseovules |
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Authors: | Faiza Tebbji André Nantel Daniel P Matton |
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Affiliation: | 1.Institut de recherche en biologie végétale, Département de sciences biologiques,Université de Montréal,Montréal,Canada;2.Biotechnology Research Institute, National Research Council, 6100 Royalmount Avenue,QC,Canada |
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Abstract: | Background To provide a broad analysis of gene expression changes in developing embryos from a solanaceous species, we produced amplicon-derived microarrays with 7741 ESTs isolated from Solanum chacoense ovules bearing embryos from all developmental stages. Our aims were to: 1) identify genes expressed in a tissue-specific and temporal-specific manner; 2) define clusters of genes showing similar patterns of spatial and temporal expression; and 3) identify stage-specific or transition-specific candidate genes for further functional genomic analyses. |
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