Comparative analysis of iron regulated genes in mycobacteria |
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Authors: | Yellaboina Sailu Ranjan Sarita Vindal Vaibhav Ranjan Akash |
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Institution: | Computational and Functional Genomics Group, Sun Centre of Excellence in Medical Bioinformatics, Centre for DNA Fingerprinting and Diagnostics, EMBnet India Node, Hyderabad 500076, India. |
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Abstract: | Iron dependent regulator, IdeR, regulates the expression of genes in response to intracellular iron levels in M. tuberculosis. Orthologs of IdeR are present in all the sequenced genomes of mycobacteria. We have used a computational approach to identify conserved IdeR regulated genes across the mycobacteria and the genes that are specific to each of the mycobacteria. Novel iron regulated genes that code for a predicted 4-hydroxy benzoyl coA hydrolase (Rv1847) and a protease dependent antibiotic regulatory system (Rv1846c, Rv0185c) are conserved across the mycobacteria. Although Mycobacterium natural-resistance-associated macrophage protein (Mramp) is present in all mycobacteria, it is, as predicted, an iron-regulated gene in only one species, M. avium subsp. paratuberculosis. We also observed an additional iron-regulated exochelin biosynthetic operon, which is present only in non-pathogenic Mycobacterium, M. smegmatis. |
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Keywords: | M tuberculosis Mycobacterium tuberculosis M avium subsp paratuberculosis Mycobacterium subsp paratuberculosis M smegmatis Mycobacterium smegmatis DtxR Diphtheria toxin repressor IdeR Iron-dependent regulator RPS-BLAST reversed position specific-basic local alignment search tool EMSA electrophoretic mobility shift assay |
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