A radioimmunocytological quantitative method for the rapid detection of ras oncogene p21 protein in mammalian cells |
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Authors: | Demetrios A Spandidos Todor Dimitrov Ephraim Y Kam |
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Institution: | (1) The Beatson Institute for Cancer Research, Garscube Estate, Bearsden, G61 1BD Glasgow, Scotland, UK;(2) Hellenic Institute Pasteur, 127 Vas, Sofias Ave, Athens, Greece |
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Abstract: | In order to provide a sensitive and quantitative detection method of ras p21 at the cytological level, the monoclonal antibody Y 13 259 and iodinated protein A were used to locate theras protein in various mammalian cell lines. The subsequent autoradiograph can be analysed by a computer-assisted system which showed in these reported experiments that the relative levels of p21 detected in these cells corresponded to results obtained earlier using conventional biochemical methods.To whom correspondence should be addressed. |
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Keywords: | Radioimmuno assay ras oncogene oncogene p21 protein |
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