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Massive isolation and identification of <Emphasis Type="Italic">Saccharomyces paradoxus</Emphasis> yeasts from plant phyllosphere
Authors:A M Glushakova  Yu V Ivannikova  E S Naumova  I Yu Chernov  G I Naumov
Institution:(1) Faculty of Soil Science, Moscow State University, Vorob’evy gory, Moscow, 119992, Russia;(2) State Research Institute of Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyi proezd 1, Moscow, 117545, Russia
Abstract:Year-round studies of epiphytic yeast communities revealed that the number of ascosporogenous yeasts of the genus Saccharomyces inhabiting living and decaying leaves of some plants increased considerably in certain short periods (at the beginning of summer and in winter). Massive isolation of saccharomycetes was performed from 11 plant species; earlier, these yeasts had been revealed mainly in sugar-rich substrates. The isolates were identified as Saccharomyces paradoxus based on their physiological properties and RELP analysis of 5.8S-ITS. Possible reasons for short-term increases in the number of saccharomycetes in plant phyllosphere are discussed.
Keywords:Saccharomyces paradoxus            phyllosphere  PCR-analysis  Moscow region
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