| 红根草的组织培养与快速繁殖研究 |
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| 引用本文： | 唐凤鸾,李锋,付传明,黄宁珍,. 红根草的组织培养与快速繁殖研究[J]. 广西植物, 2006, 26(3): 282-285 |
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| 作者姓名： | 唐凤鸾 李锋 付传明 黄宁珍 |
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| 作者单位： | 广西壮族自治区,中国科学院,广西植物研究所,广西,桂林,541006;广西壮族自治区,中国科学院,广西植物研究所,广西,桂林,541006;广西壮族自治区,中国科学院,广西植物研究所,广西,桂林,541006;广西壮族自治区,中国科学院,广西植物研究所,广西,桂林,541006 |
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| 基金项目： | 广西科技攻关项目(桂科攻0322024-3B)~~ |
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| 摘 要： | 研究了红根草不同采集季节、外植体类型与消毒效果,不同激素浓度和激素组合与芽及根的诱导,及试管苗的移栽。结果表明,不同外植体类型消毒效果为茎节>茎尖>植株抽茎前的生长点;4~6月份采样最佳;MS+BA0.8~1.6mg/L+NAA0.2mg/L、MS+BA0.2mg/L+NAA0.02mg/L、MS+NAA1.0mg/L分别用于初代、继代、生根培养效果最好;试管苗移栽成活率达90%。
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| 关 键 词： | 红根草 组织培养 快速繁殖 |
| 文章编号： | 1000-3142(2006)03-0282-04 |
| 收稿时间： | 2005-02-22 |
| 修稿时间： | 2005-10-23 |
Tissue culture and rapid propagation of Salvia prionitis |
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| TANG Feng-luan,LI Feng,FU Chuan-ming,HUANG Ning-zhen. Tissue culture and rapid propagation of Salvia prionitis[J]. Guihaia, 2006, 26(3): 282-285 |
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| Authors: | TANG Feng-luan LI Feng FU Chuan-ming HUANG Ning-zhen |
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| Affiliation: | Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Academia Sinica, Guilin 541006, China |
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| Abstract: | Tissue culture and rapid propagation of Salvia prionitis were reported in this paper. Effects of several factors such as different explants,collecting periods on the influence of sterilization, different phytohormones concentration,medium on the induction of buds and roots,and transplantation of seedlings were studied. Results showed that stem was the best explant for sterilization,the next one was the tip of stem and the third one was the low tip on the ground before the stem outgrowth. The best collecting periods of explants was from April to June. The most suitable medium for bud induction was MS+BA 0.8～1.6 mg/L+NAA 0.2 mg/L,MS+BA 0.2 mg/L+NAA 0.02 mg/L was optimum for proliferation, and MS+NAA1.0 mg/L was best for root induction. The survival rate of the seedlings was about 90%. |
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| Keywords: | Salvia prionitis tissue culture rapid propagation |
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