Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines |
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| Authors: | Sarath K. Kalapala Sven N. Hobbie Erik C. B?ttger Dmitry Shcherbakov |
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| Affiliation: | 1. Institut für Medizinische Mikrobiologie, Universität Zürich, Zürich, Switzerland.; 2. Singapore-MIT Alliance for Research and Technology (SMART), Centre for Life Sciences, Singapore, Singapore.;University of Delhi, India |
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| Abstract: | Recent studies have suggested that ribosomal protein S12 modulates 16S rRNA function and susceptibility to 2-deoxystreptamine aminoglycosides. To study whether the non-restrictive K42R mutation in RpsL affects 2-deoxystreptamine susceptibility in Mycobacterium smegmatis, we studied the drug susceptibility pattern of various mutants with genetic alterations in the 16S rRNA decoding A-site in the context of wild-type and mutant protein S12. RpsL K42R substitution was found not to affect the drug resistance pattern associated with mutational alterations in 16S rRNA H44. |
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