Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS |
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Authors: | Honggang Nie Ranran Liu Youyou Yang Yu Bai Yafeng Guan Daqing Qian Tao Wang Huwei Liu |
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Affiliation: | 1. Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China;2. Ministry of Education (MOE) Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Peking University, Beijing, China;4. Institute of Analytical Chemistry, Peking University, Beijing, China;7. Department of Instrumental and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China;11. Department of Medicine, Wannan Medical College, Wuhu, Anhui, China;8. Division of Nephrology, Peking University Third Hospital, Beijing, China |
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Abstract: | An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivity. Different lipid classes were separated on a normal-phase column in the first dimension and lipid molecular species were separated on a reversed-phase column in the second dimension, so that the ion suppression effects were reduced while the detection sensitivity was improved. Identified were 721 endogenous lipid species from 12 lipid classes, in which 415 structures were confirmed using tandem mass spectra, and the other 306 lipid molecular species were identified by accurate masses. The linearity, limit of detection, and repeatability were all satisfactory. The method was applied to the investigation of the lipid changes in rat peritoneal surface layer after peritoneal dialysis, and 32 potential lipid biomarkers were identified, as their concentrations in the dosed group were 2.2–12.5 times of those in the control group. The results revealed that this 2D LC-MS system was a promising tool for lipid profiling of complex biological samples. |
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Keywords: | lipidomic free fatty acid phospholipid lyso-phospholipid plasmalogen peritoneal dialysis peritoneum biomarker two-dimensional liquid chromatography mass spectrometry |
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