A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from a marine Chlorella ellipsoidea and its antihypertensive effect in spontaneously hypertensive rats |
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Authors: | Seok-Chun Ko Nalae Kang Eun-A Kim Min Cheol Kang Seung-Hong Lee Sung-Myung Kang Joon-Baek Lee Byong-Tae Jeon Se-Kwon Kim Sun-Joo Park Pyo-Jam Park Won-Kyo Jung Daekyung Kim You-Jin Jeon |
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Institution: | 1. Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea;2. Department of Earth Marine Sciences, Jeju National University, Jeju 690-756, Republic of Korea;3. Korean Nokyong Research Center, College of Medicine Konkuk University, Chungju 380-701, Republic of Korea;4. Department of Chemistry, Pukyoung National University, Busan 608-737, Republic of Korea;5. Department of Biotechnology, College of Medicine Konkuk University, Chungju 380-701, Republic of Korea;6. Department of Marine Life Science, Marine Life Research center, Chosun University, Gwangju 501-759, Republic of Korea;7. Marine Bio Research Team, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea |
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Abstract: | Marine Chlorella ellipsoidea protein was hydrolyzed using Protamex, Kojizyme, Neutrase, Flavourzyme, Alcalase, trypsin, α-chymotrypsin, pepsin and papain. Alcalase-proteolytic hydrolysate exhibited the highest ACE inhibitory activity among them and was fractionated into three ranges of molecular weight (below 5 kDa, 5–10 kDa and above 10 kDa). The below 5 kDa fraction showed the highest ACE inhibitory activity and was used for subsequent purification steps. During consecutive purification, a potent ACE inhibitory peptide from marine C. ellipsoidea, which was composed of 4 amino acids, Val–Glu–Gly–Tyr (MW: 467.2 Da, IC50 value: 128.4 μM), was isolated. Lineweaver–Burk plots suggest that the peptide purified acts as a competitive inhibitor against ACE and stable against gastrointestinal enzymes of pepsin, trypsin and α-chymotrypsin. Furthermore, antihypertensive effect in spontaneously hypertensive rats (SHRs) also revealed that oral administration of purified peptide can decrease systolic blood pressure significantly. The results suggest that marine C. ellipsoidea would be an attractive raw material for the manufacture of antihypertensive nutraceutical ingredients. |
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