Effect of microwave irradiation on the structure of glucoamylase |
| |
Authors: | Xiaoyun Zhang Tianliang Cao Xueda Tian Dongying Gai |
| |
Institution: | School of Chemical Engineering, Xiangtan University, Hunan, Xiangtan 411105, PR China |
| |
Abstract: | In the present study, we describe changes in the primary and secondary structural patterns of glucoamylase during starch hydrolysis under microwave irradiation using SDS-PAGE and circular dichroism (CD) spectroscopy. Our SDS-PAGE results show that the primary structure of glucoamylase did not change after microwave irradiation. According to the CD spectra, the positive peak height (λ = 193 nm) of the microwave-irradiated samples decreased by 36.4–68.2% compared to those without irradiation, whereas the double negative peak height (λ = 206 nm, λ = 220 nm) increased by 10.8–31.4%. In addition, the positive peak (λ = 193 nm) shifted by 0.2–3 nm. After treatment of glucoamylase with microwave irradiation, the α-helical content of glucoamylase decreased sharply, whereas the β-sheet, β-turn and random coil content increased gradually. The conformational changes of glucoamylase after microwave irradiation provide theoretical support for the mechanism whereby microwave irradiation accelerates starch hydrolysis catalyzed by glucoamylase. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|