Immobilization of glucose oxidase onto cobalt based on silica core/shell nanoparticles as carrier |
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Authors: | Hee Uk Lee Chulhwan Park Seung Wook Kim |
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Institution: | 1. Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Republic of Korea;2. Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-Dong, Nowon-Gu, Seoul 139-701, Republic of Korea |
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Abstract: | Co–B/SiO2/NH2 magnetic nanoparticles (NPs) were prepared from a silica shell-coated Co–B core using the Stöber method and amine-modification on the surface. Glucose oxidase (GOD) was covalently immobilized on the surface of Co–B/SiO2/NH2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The magnetic NPs characteristics, such as the synthesis of Co–B/SiO2/NH2 NPs, effect of pH, temperature, and concentration of buffer for enzyme immobilization, were investigated. The optimal reaction conditions for immobilization were determined to be 0.1 M of phosphate buffer solution, pH 7.0, and 5 °C. In the case of immobilized GOD without d-glucose and with 0.1 M of d-glucose for blocking, 22.98 U/g and 24.83 U/g of their original activity were retained after 7 reuses, respectively. |
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