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Spectrophotometric estimation of cobalt with ninhydrin
Authors:Karamat Mahmood  Feroza Hamid Wattoo  Muhammad Hamid Sarwar Wattoo  Muhammad Imran  Muhammad Javaid Asad  Syed Ahmed Tirmizi  Abdul Wadood
Institution:1. Department of Chemistry, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan;2. Institute of Biochemistry & Biotechnology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan;3. Department of Chemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan
Abstract:A violet coloured complex was developed when cobalt metal reacts with ninhydrin at pH 8.2, using sodium acetate buffer solution. Absorbance of the complex was measured at 395 nm. Various factors, such as volume of the ligand used, solution pH, stability of the complex with time and interference of other metals, which effect the complex formation have been studied in detail. Present developed method can be used for the spectrophotometric estimation of cobalt with ninhydrin complex. The method is simple, selective and cheap for the determination of cobalt in very less time.
Keywords:Spectrophotometric estimation  Cobalt  Ninhydrin
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