Astaxanthin production by a highly photosensitive Haematococcus mutant |
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| Authors: | Min-Eui Hong Seung Phill Choi Youn-Il Park Young-Kee Kim Won Seok Chang Byung Woo Kim Sang Jun Sim |
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| Institution: | 1. Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, South Korea;2. Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, South Korea;3. Department of Biology, Chungnam National University, Daejeon 305-764, South Korea;4. Division of Chemical Engineering, Hankyong National University, 327 Joongang-Ro, Anseong, Gyeonggi-Do 456-749, South Korea;5. Research Institute, Korea District Heating Corp., 186 Bundang-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, South Korea |
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| Abstract: | Haematococcus pluvialis synthesizes a high yield of astaxanthin using CO2 in a photoautotrophic culture without contaminant heterotrophs; however, it takes too long to induce astaxanthin production. In this study, a highly photosensitive mutant strain was attained by conventional random mutagenesis and an efficient isolation method to shorten induction time. Sensitivity to photoinhibition in this mutant was raised by a partial lesion in the photosystem II (PSII) of photosynthesis, thereby prompting a change in cellular morphology as well as stimulating carotenogenesis (astaxanthin production). As a result, the concentrations of cell biomass and astaxanthin were dramatically increased by 27% and 62% under strong light and 79% and 153% under moderate light, respectively. This Haematococcus mutant would be useful for the economical astaxanthin production capable of reducing the light energy cost in a photoautotrophic culture system, even in areas with insufficient sunlight. |
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