Genetic fingerprinting for determining the mode of reproduction in Paspalum notatum, a subtropical apomictic forage grass |
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Authors: | J P A Ortiz S C Pessino O Leblanc M D Hayward C L Quarín |
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Institution: | (1) Institute of Grassland and Environmental Research (IGER), Plas Gogerddan, Aberystwyth, Ceredigion, SY23 3EB, UK Fax: +44 1970 828357 e-mail: juanpa.ortiz@bbsrc.ac.uk, GB;(2) Instituto de Botánica del Nordeste (IBONE) – Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, CC 209, 3400 Corrientes, Argentina, AR |
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Abstract: | Paspalum is an important genus of the family Gramineae that includes several valuable forage grasses. Many of the species are polyploid
and either obligate or facultative apomicts. Cyto-embryological observations of several tetraploid genotypes of P. notatum were performed to determine their mode of reproduction. Afterwards, selfed progenies of the genotypes F131, Q3664 and Q4117
were analysed using RFLP and RAPD genetic fingerprints to identify maternal and non-maternal (aberrant) plants, and to establish
the degree of apomictic reproduction. Five maize clones and six primers were used for detecting genetic deviations from the
maternal profile. Maize clones umc379, umc384 and umc318 and primers OPG10 and OPI4 were the most informative for discriminating
between maternal and aberrant individuals within the progenies of F131 and Q3664. The combined results of three RFLP clones
or 4–6 RAPD primers were necessary to ascertain the mode of reproduction in plants F131 and Q3664. The results obtained with
the RFLP and RAPD markers were in agreement with the cyto-embryological studies in ascertaining the mode and degree of apomictic
reproduction. Plant F131 showed a completely sexual reproductive behaviour, Q3664 an elevated expression of sexuality, while
Q4117 was highly apomictic. A fingerprint analysis of an outcrossing population, aimed at the identification of hybrid plants,
was also performed. Maize clones um318 and umc379 and primers OPC2 and OPC9 were used. The presence of specific bands belonging
to the male parent permitted a rapid and easy detection of hybrids. The methodology described here can be applied both for
the characterisation of P. notatum populations and to identify hybrid progenies in Paspalum breeding programs.
Received: 5 March 1997 / Accepted: 13 May 1997 |
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Keywords: | Apomixis DNA fingerprinting Molecular markers Paspalum Progeny test |
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