HZ-841吸附树脂精制银杏叶总黄酮

本文研究了用HZ-841吸附树脂精制银杏叶总黄酮的工艺。用10 BV 70％的乙醇分三次提取脱脂银杏叶粉中的银杏叶总黄酮，其收得率为4．8％，纯度为21．7％；用30BV纯净水、微波解冻提取30min，银杏叶总黄酮的收得率及纯度分别是2．63％和13．4％。HZ-841树脂对银杏叶总黄酮的动态吸附容量在pH=7．0时为0．265g／mL，树脂，动态吸附平衡时间为10min。酸度对HZ-841树脂吸附银杏叶总黄酮有显著影响，当pH=5．0时，其静态吸附量可达到0．322g／mL。吸附了银杏叶总黄酮的HZ-841树脂可用乙醇洗脱，当洗脱液pH=9．0、乙醇浓度为90％、洗脱流速3BV／h时，5BV洗脱液的收得率为1．8％。用无水乙醇洗脱的银杏叶总黄酮经过真空浓缩、干燥，获得的浅黄色粉末中银杏叶总黄酮含量为37．3％，产品收得率为2．41％。

Purification of Total Flavones of Ginkgo biloba L. Leaves by HZ-841 Adsorption Resin

It was researched that processing of purification total flavones of Ginkgo biloba L.leaves by HZ-841 adsorption resin and the experiment result was as followed.The yield is 4.8%,and the purity 21.7%. While the flavones from a defat powder of Ginkgo biloba L.leaves was extracted by 10 BV alcohol which concentration was 70% and by three times,therefore,the yield or the purity is 2.63% and 13.4% respectively using 30 BV water and microwaves that treatment time was 30 min at unfreezing gear.The dynamic exchange capacity is 0.265 g/mL resin and the equilibrium time 10 min respectively at pH 7.0.The adsorption capacity was effected marked by hydrogen concentration and at pH 5.0 the static adsorption capacity is arrived to 0.322 g/mL resin.Total flavones of Ginkgo biloba L.leaves which was adsorbed by HZ-841 resin could be eluted by alcohol,and the yield is 1.8% when pH was equal to 9.0,alcohol concentration 90%,eluting rate 3 BV/h and volume 5 BV.A yellow powder of the total flavones was obtained through alcohol elution,vacuum enrichment and dry,but the yield is 2.41%,the total flavones concentration 37.3% in it.