Specific binding of endotoxin to human monocytes and mouse macrophages: serum requirement |
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| Authors: | N Haeffner-Cavaillon J M Cavaillon M Etievant S Lebbar L Szabo |
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| Affiliation: | 1. Equipe 55 du CNRS, Institut de Biochimie, Université de Paris XI, 91405 Orsay France;2. Unité d''Immuno-Allergie, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France;1. Institut Pasteur, 75015 Paris, France;2. Pfizer, 75014 Paris France;3. CNRIPH, ministère de la Santé, 75007 Paris, France;4. Inserm, 75654 Paris, France;5. AstraZeneca France, 92400 Courbevoie, France;6. AP–HP, Hôpital Antoine Béclère, 92140 Clamart, France;7. Hôpital Nord - CHU de Saint-Étienne, 42270 St Priest en Jarez, France;8. SC Conseil, 92400 Courbevoie, France;9. Hôpital Michallon, CHU de Grenoble-Alpes, 38700 La Tronche, France;10. Institut Paoli-Calmettes, 13009 Marseille, France;11. Sanofi R&D, 69360 Serezin du Rhône, France;12. GSK France, 92500 Rueil-Malmaison, France;13. University of Oxford, United Kingdom; Monash University, University of Melbourne, Melbourne, Australie;14. Université Paris Cité; AP–HP, CIC vaccinologie Cochin Pasteur–Hôpital Cochin; Inserm, FCRIN, I REIVAC, 75014 Paris France;15. Université Paris Cité; AP–HP, Ateliers de Giens, Hôpital Cochin, 75014 Paris, France;p. Galien Affaires, 75014 Paris, France;1. Institut Pasteur, 75015 Paris, France;2. Pfizer, 75014 Paris, France;3. CNRIPH, ministère de la Santé, 75007 Paris, France;4. Inserm, 75654 Paris, France;5. AstraZeneca France, 92400 Courbevoie, France;6. AP–HP, hôpital Antoine-Béclère, 92140 Clamart, France;7. Hôpital Nord, CHU St-Étienne, 42270 St Priest en Jarez, France;8. SC Conseil, 92400 Courbevoie, France;9. Hôpital Michallon, CHU Grenoble-Alpes, 38700 La Tronche, France;10. Institut Paoli-Calmettes, 13009 Marseille, France;11. Sanofi R&D, 69360 Serezin du Rhône, France;12. GSK France, 92500 Rueil-Malmaison, France;13. University of Oxford, United Kingdom; Monash University, University of Melbourne, Melbourne, Australia;14. Université Paris Cité, AP–HP, CIC vaccinologie Cochin Pasteur, hôpital Cochin, Inserm, FCRIN, I REIVAC, 75014 Paris, France;15. Université Paris Cité, AP–HP, Ateliers de Giens, hôpital Cochin, 75014 Paris, France;p. Galien Affaires, 75014 Paris, France;1. Institut Pasteur, Université Paris Cité, CNRS UMR6047, INSERM U1306, Unité de Biologie et Génétique de la Paroi Bactérienne, Paris, France;2. Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité Biologie des Bactéries Intracellulaires, Paris, France |
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| Abstract: | Specific binding of Bordetella pertussis and Neisseria meningitidis endotoxins to human monocytes and murine macrophages was demonstrated. Binding of B. pertussis endotoxin could be inhibited by endotoxins of Salmonella minnesota, Escherichia coli, and Klebsiella pneumoniae, the extent of inhibition being dependent on the origin of the lipopolysaccharides and on the origin of the mononuclear phagocytic cells. The binding of B. pertussis and N. meningitidis endotoxins which was mediated by the polysaccharide region of the endotoxins was serum dependent. The results indicated that the binding of endotoxin was promoted neither by natural antibodies directed against the endotoxin nor by proteins known to combine with endotoxins: immunoglobulins, albumin, or fibronectin; we have provided some evidence that complement components may play a role in the specific binding of endotoxins to the monocyte/macrophage membrane. |
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