Localization of 5S DNA by in situ hybridization in metaphase chromosomes of Vicia faba L.

The tritium-labelled cloned 5S DNA from Lupinus luteus was used for localization of 5S RNA genes in Vicia faba subsp. minor metaphase chromosomes. In situ hybridization sites were found to be localized in chromosomes I and VI. In chromosome I the probe hybridized to the region adjoining NOR whereas in chromosome VI silver grains were found in the median part of the long arm. After prolonged exposure the autoradiographic grains expanded in the proximal part of that chromosome arm.