Characterization of AT2 Receptor Expression in NIH 3T3 Fibroblasts |
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| Authors: | Frank M. J. Heemskerk Stefan Zorad Ningzhi Xu Silvio J. Gutkind Juan M. Saavedra |
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| Affiliation: | (1) Section on Pharmacology, National Institute of Mental Health, 10 Center Drive, Building 10/2D57, Bethesda, Maryland, 20892-1264;(2) Laboratory of Cellular Development and Oncology, National Institute of Dental Research, Bethesda, Maryland, 20892 |
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| Abstract: | 1. A high expression of angiotensin II receptors and of angiotensin-converting enzyme (ACE) activity was detected in confluent NIH 3T3 fibroblasts.2. Characterization with selective ligands, dithiothreitol, and GTP S, indicated that only the AT2 subtype was expressed.3. AT2 receptors and ACE expression were strictly dependent on the cell density and growth phase of the cells, with AT2 receptors being expressed earlier than ACE. In contrast, high expression of AT2 receptors irrespective of their growth state was observed in NIH 3T3 cells lacking contact inhibition upon neoplastic transformation with ras.4. Our results imply a possible relation of AT2 receptors to cell growth and cell–cell contact. |
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| Keywords: | AT2 receptor growth curve cell– cell contact angiotensin converting enzyme NIH3T3 fibroblasts neoplastic transformation |
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