Electrochemical analysis of blood cell/substrate interactions under flow conditions |
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| Institution: | 2. Laboratoire de Biorhéologie et d''Hydrodynamique Physicochimique, CNRS URA 343, Université Paris 7, 2, Place Jussieu, 75251 Paris Cedex 05, France;3. Laboratoire d''Energétique, Ecole Centrale de Nantes, 1 rue de la Noë, 44072, Nantes Cedex 03, France;1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China;2. School of Life Sciences, Shanghai University, Shanghai, 200444, China;3. Department of Orthopedic Surgery, Brigham and Women''s Hospital, Harvard Medical School, Boston, MA, 02115, USA;4. Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai, 200233, China;1. Department of Engineering, Aarhus University, Gustav Wiedsvej 10C, DK-8000 Aarhus C, Denmark;2. Department of Biology, Università di Roma Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy;3. Department of Chemistry and iNANO, Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark;1. Department of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;2. Department of General Surgery, Xiangyang Central Hospital, Hubei University of Arts and Science, Xiangyang 441021, China |
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| Abstract: | Interactions of blood cells (RBCs) with a microelectrode of 50 (im diameter have been examined under flow conditions using impedance measurements at high frequencies. At such frequencies, the electrolyte resistance (Re,) is assimilated to the real part of impedance, and interactions are associated with transient fluctuations of Re. Sedimentation experiments suggest that one erythrocyte contributes to a 1.1% Re, increase. Effects of wall shear rate (from 25 to 140 s1) and RBC concentration (from 8.4 × 105 to 2.7 × 106 cells/ml) have been investigated; the number of interactions rapidly decreases with wall shear rate. Event frequency is proportional to RBC concentration ranging from 3.1 × 106 cells/ml to 1.3 × 107 cells/ml. At high concentrations of RBCs, some transient events overlap. Videotaped images help to determine how many RBCs interact with the microelectrode at the same time on separate surface areas. Under flow conditions, the contribution of one RBC on the Re increase is similar to the mathematical value obtained by sedimentation and decreases slightly with wall shear rate. |
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